1.Key Laboratory of Optical Information Detection and Display Technology of Zhejiang, Zhejiang Normal University;2.Centre for Optical and Electromagnetic Research,Zhejiang University
The National Natural Science Foundation of China,Fundamental Research Funds for the Central Universities,Zhejiang Provincial Natural Science Foundation of China
本文基于光学手段，开发出了一套能够兼顾可见光与近红外二区（NIR-II）窗口的双通道宏观成像系统，能够实时输出具有NIR-II荧光信息的高质量明场图像，解决了常规的NIR-II宏观成像系统提供的荧光图像与明场图像割裂较大的问题。实验中利用不同厚度的脂肪组织测试了吲哚菁绿的NIR-II窗口荧光信号分别在1100-1700 nm长通滤光片与1300-1700 nm长通滤光片下的抗散射能力与成像表现。接着利用所搭建的双通道宏观成像系统分别在小鼠以及大鼠模型上实现了淋巴结成像并模拟进行了淋巴结的切除手术，模拟了对腹腔淋巴结清扫的过程。最后，在大鼠模型上添加了不同厚度的生物脂肪组织，模拟实际手术中脂肪组织覆盖在淋巴结上的情况，观察双通道系统的穿透能力。可见近红外二区双通道荧光成像系统通过给操作者提供直观的影像信息，缩减手术时间、提高患者的预后，在临床手术导航中具有较大的应用潜力。
A dual-channel macroscopic imaging system has been developed based on optical methods, which can simultaneously capture the visible light and near-infrared second window (NIR-II) fluorescence. It could provide high-quality bright-field real-time images with the NIR-II fluorescence information, addressing the significant disparity issue between fluorescence and bright-field images in conventional NIR-II macroscopic imaging systems. In the experiment, the anti-scattering capability and imaging performance of NIR-II fluorescence signals of indocyanine green (ICG) were tested using different thicknesses of adipose tissues in the band of 1100-1700 nm and 1300-1700 nm respectively. Subsequently, the dual-channel macroscopic imaging system was used to obtain lymph node images of mouse and rat models, simulating the lymph node resection surgery and mimicking the process of abdominal lymph node clearance. Finally, different thicknesses of biological adipose tissues were added to the rat model to simulate the presence of adipose tissues covering the lymph nodes during actual surgery, and the penetration capability of the dual-channel system was observed. The visible and near-infrared second window dual-channel fluorescence imaging system provided the intuitive visual information to the operator, reducing surgery time and improving the patient prognosis, and held great potential for application in clinical surgical navigation.