The feasibility of using an infrared spectral analysis technology to detect cell disruption in the process of electric transfection is studied. Electric transfection is a means commonly used in genetic engineering experiments. It usually uses an electroporation method to incorporate the genes outside a cell into the cell and let them express. In the process of electric transfection, a cell may be perforated and damaged due to the stimulation of strong external voltage. The infrared spectra of porcine kidney cells are obtained before and after their damage respectively. The spectral data in the region from 2200 cm-1 to 2600 cm-1 are analyzed through second derivation combined with a computer software. It is found that the characteristic peaks of the cells before and after their damage are very different in this spectral region. The shape of the characteristic peak of the damaged cell is sharpen and intensive while the shape of the characteristic peak of the normal cell is wider and its intensity is low. This result provides the basis for the identification of cell damage and is of reference value to the further use of infrared spectroscopy in cell damage research.