A near-infrared spectral analysis model is established by using a multi-model method. The model can be used to rapidly determine the lignin content in populus euramericana. The correlation coefficient between the predicted value and the experimental value is 0.9702. The mean relative error of the prediction is 0.0100. The goodness of the fit model is 0.9126. Compared with the determination model established by using the partial least squares (PLS) method, the prediction of this model is better.