The cryocooler is the key component of Stirling refrigerator, its parameters directly affect the working efficiency of the refrigerator, and the stainless steel wire mesh is the filler of regenerator. In this paper, the influence of wire mesh parameters on the performance of Stirling refrigerator is analyzed. The flow field distribution and pressure distribution in the regenerator are obtained by computational fluid dynamics (CFD) simulation. The relationship between the void ratio and flow rate is obtained by means of flow measurement system. The larger the void ratio is, the larger the internal flow rate of the regenerator is, and the cooling time increases. The effect of different void ratios and numbers of meshes on the performance is studied by experiments. The results show that when the number of meshes is the same and the void ratio of regenerator is 0.61－0.78, the cooling time of refrigerator increases with the increase of void ratio. When the number of regenerator wire meshes is 600－800, the cooling time of the refrigerator decreases with the increase of the number of regenerator wire meshes filling.