The mechanism of stripe noise was analyzed. An algorithm for eliminating stripe noise in one frame time was proposed. The algorithm obtains the extreme value of every row pixels by traversing all pixels of a frame in local window and computes noise gain and offset parameters through the succession of adjacent row pixels. By setting adaptive threshold value, eliminating the influences of image edges and system noise, we can achieve the precise gain and offset parameters and complete the eliminating algorithm for stripe noise. The experiment and theoretical analysis indicate that our algorithm can eliminate stripe noise effectively in one frame time.