Fluorescence imaging in the second near-infrared window （NIR-II， 900-1880 nm） offers high signal-to-background ratio （SBR）， enhanced definition， and superior tissue penetration， making it ideal for real-time surgical navigation. However， with single-channel imaging， surgeons must frequently switch between the surgical field and the NIR-II images on the monitor. To address this， a coaxial dual-channel imaging system that combines visible light and 1100 nm longpass （1100LP） fluorescence was developed. The system features a customized coaxial dual-channel lens with optimized distortion， achieving precise alignment with an error of less than ±0.15 mm. Additionally， the shared focusing mechanism simplifies operation. Using FDA-approved indocyanine green （ICG）， the system was successfully applied in dual-channel guided rat lymph node excision， and blood supply assessment of reconstructed human flap. This approach enhances surgical precision， improves operational efficiency， and provides a valuable reference for further clinical translation of NIR-II fluorescence imaging.