The study of selection with He-Ne laser or He-Ne laser plus lithium chloride in streptomyces rimosus is reported. Under identical experiment conditions, the irradiation with He-Ne laser is better than that with He-Ne laser plus lithium chloride. After irradia- tion with He-Ne laser, experimental high-production strain 5221 was obtained. Its unit of fermentation was increased by 5% as compared with the control.