近红外光谱光纤探头的创新设计用于骨关节炎的原位检测
作者:
作者单位:

南京航空航天大学 自动化学院 生物医学工程系,江苏 南京 211106

作者简介:

通讯作者:

中图分类号:

O433

基金项目:

国家自然科学基金(61378087),江苏省自然科学基金(BK20151478)


Novel design of a NIR fiber optic probe for in situ detection of osteoarthritis
Author:
Affiliation:

Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics,Nanjing 211106,China

Fund Project:

Supported by National Natural Science Foundation of China (61378087), Natural Science Foundation of Jiangsu Province(BK20151478)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    创新设计了一款操作灵活、方便、适于生物医学原位检测的反射式近红外(NIR)光纤探头,通过将自聚焦透镜耦合到NIR光纤探头的顶端,并对探头结构、光纤排布进行全新设计,使光纤探头具有更高的测量精度和收集效率。通过耦合傅里叶变换近红外光谱仪对蔗糖样本进行NIR光谱采集,发现该反射式NIR光纤探头具有高效便捷的特点以及较高的光谱重复性和信噪比。采用该光纤探头对犬膝关节股骨端关节软骨进行NIR光谱离体原位检测,这些光谱数据经一阶导数2次多项式21点Savitzky-Golay平滑预处理后再进行主成分分析和Fisher判别分析。模型初始案例和交互验证案例正确识别率分别为97.62%、90.47%,样本预测集的识别率达96.43%,证明了采用该NIR光纤探头进行NIR光谱原位检测的有效性及骨关节炎识别的可行性,可为骨关节炎的临床诊断奠定实验基础。

    Abstract:

    An innovative reflective near-infrared (NIR) fiber probe with flexibility and convenience was designed for biomedical in-situ detection. When the brand-new fiber probe structure and fiber arrangement were designed, further, a gradient-index (grin) lens was coupled to the top of the reflective NIR fiber bundles to make it have higher measurement accuracy and collection efficiency. When measuring NIR spectra of sucrose samples by coupling the grin NIR fiber probe to Fourier transform near infrared spectrometer, it was found that the grin NIR fiber probe had the advantages of convenience, high efficiency and spectral repeatability and SNR. The NIR spectra of articular cartilage at the femoral end of the canine knee joint were measured in situ by the grin NIR fiber probe and were preprocessed by first-derivative quadratic polynomials 21-point Savitzky-Golay smoothing for principal component analysis and Fisher discrimination analysis (PCA-FDA). The correct recognition accuracies of PCA-FDA model in initial cases and cross validation cases were 97.62% and 90.47%, respectively, as well as 96.43% for the prediction set, which confirms the effectiveness of NIR fiber probe detection in situ and the feasibility of osteoarthritis recognition and lays the foundation for basic research and early clinical diagnosis of osteoarthritis.

    参考文献
    相似文献
    引证文献
引用本文

符娟娟,唐金兰,包一麟,尚慧,吴进锦,王潇,尹建华.近红外光谱光纤探头的创新设计用于骨关节炎的原位检测[J].红外与毫米波学报,2021,40(6):784~790]. FU Juan-Juan, TANG Jin-Lan, BAO Yi-Lin, SHANG Hui, WU Jin-Jin, WANG Xiao, YIN Jian-Hua. Novel design of a NIR fiber optic probe for in situ detection of osteoarthritis[J]. J. Infrared Millim. Waves,2021,40(6):784~790.]

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2020-11-09
  • 最后修改日期:2021-08-01
  • 录用日期:2021-03-17
  • 在线发布日期: 2021-07-30
  • 出版日期: