A high-speed infrared video acquisition system is proposed. The design and implementation of a PCI-E acquisition board which is the key module of the acquisition system is mainly presented. The acquisition board is mainly based on the hardware structure of Kintex7 Field-Programmable Gate Array (FPFA). In the acquisition system, the high-speed fiber transmission and PCI-E technology are used successfully by utilizing Aurora core and Xilinx 7 series PCI-E IP core. The experimental results show that this high-speed infrared video acquisition system can operate stably and reliably for a long time. It is very suitable for the real-time acquisition, display and storage of high frame rate and large array infrared video.