In order to meet the requirements of data acquisition, nonlinear fitting compensation, signal preprocessing and Fourier spectrum inversion of transmission rotary mirror Fourier spectrometer, a set of transmission rotary mirror Fourier spectrum analysis software integrating data acquisition and signal analysis is designed based on LabVIEW. By calling the physical channel interface of NI data acquisition card, the software collects the interferogram data of the amplitude of interference signal changing with time, and restores the spectrum information of infrared signal by signal processing program. It can realize automatic storage of waveform files and visualization of important data processing processes, which is suitable for industrial computer applications. The spectral curve of blackbody radiation source is measured. The wavelength accuracy is measured by polystyrene absorption spectrum, and the spectral half-height width is measured by water vapor spectrum curve. By using the spectral analysis software designed in this paper, the spectral distribution of infrared signal is recovered. The wavelength accuracy of 0.40% and the spectral resolution of 0.88 cm^-1 are achieved, which verifies the usability of the system.