Infrared Atmospheric Sounding Interferometer (IASI) can be used to acquire stratospheric temperature profiles and analyze stratospheric gravity waves. On the basis of the neural network, a formula for IASI to retrieve the stratospheric temperature profiles in the altitude range from 25 km to 60 km is established by using the typical atmospheric profile database and the atmospheric radiation transfer mode. The retrieval results are verified according to the reanalysis results. The simulation test results show that the retrieval deviations of stratospheric temperatures are mainly close to 0 K and are not greater than 1 K. The root mean square error is between 2 K and 6 K and the root mean square error in the range from 50 hPa to 5 hPa is within 3 K. The comparison results show that the retrieval of temperature profiles from IASI_SFOV is comparable to the reanalysis data from ECMWF on the whole. The retrieval is faster and has a more complete coverage area.